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Two size classes of O-glycosidically linked oligosaccharides were liberated from glycoprotein E1 of mouse hepatitis virus (MHV) A59 by reductive beta-elimination and separated by h.p.l.c. The structures of the reduced oligosaccharides were determined by successive exoglycosidase digestions and by methylation analyses involving combined capillary gas chromatography-mass spectrometry and mass fragmentography after chemical ionization with ammonia. Oligosaccharide A (Neu5Ac alpha 2----3 Gal beta 1----3 GalNAc) comprised 35% of the total carbohydrate side chains, while the remaining 65% of the oligosaccharides of E1 had the branched structure B: Neu5Ac alpha 2----3 Gal beta 1----3 (Neu5Ac alpha 2----6) GalNAc. Both oligosaccharides were linked to the E1 polypeptide via N-acetylgalactosamine, and 20% of the sialic acids present in E1 glycopeptides were found to consist of N-acetyl-9-mono-O-acetylneuraminic acid. The reported structures of the O-linked glycans are discussed in the context of the amino acid sequence of E1, which exhibits a cluster of four hydroxyamino acids (Ser-Ser-Thr-Thr) as potential O-glycosylation sites at the amino terminus. Oligosaccharides with identical structures and an identical O-glycosylated tetrapeptide sequence are present in the blood group M-active glycophorin A of the human erythrocyte membrane.
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
October 1983,
The Journal of biological chemistry,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
January 1987,
Advances in experimental medicine and biology,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
October 1985,
Journal of virology,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
June 1989,
European journal of biochemistry,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
February 1981,
The Journal of biological chemistry,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
August 1988,
The Journal of biological chemistry,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
September 2020,
Immunology letters,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
May 1988,
The Journal of cell biology,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
January 1980,
Microbiology and immunology,
H Niemann, and
R Geyer, and
H D Klenk, and
D Linder, and
S Stirm, and
M Wirth
April 1992,
Glycoconjugate journal,
