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A 1,4-dihydroypyridine arylazide photoaffinity ligand, [3H]azidopine (50.6 Ci/mmol), has been synthesized. [3H]Azidopine binds reversibly with a Kd of 350 pM to guinea-pig skeletal muscle membranes in the absence of ultraviolet light. The reversible [3H]azidopine binding is inhibited steroselectively by 1,4-dihydropyridines, phenylalkylamine Ca2+ channel blockers and La3+. Covalent incorporation into membrane proteins after photolysis was investigated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis. [3H]Azidopine is photoincorporated specifically into a protein of Mr approximately 145 000. The covalent labelling of the Mr approximately 145 000 band is inhibited stereoselectively by drugs and cations which block the reversible [3H]azidopine binding. It is suggested that [3H]azidopine is photoincorporated into a subunit of the putative Ca2+ channel.
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
August 1985,
The EMBO journal,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
July 1989,
Biochemical and biophysical research communications,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
January 1986,
Journal of steroid biochemistry,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
December 1986,
FEBS letters,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
December 1991,
Proceedings of the National Academy of Sciences of the United States of America,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
April 1987,
The Biochemical journal,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
April 1994,
General physiology and biophysics,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
April 1988,
Biochemical pharmacology,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
August 1992,
Glycobiology,
D R Ferry, and
M Rombush, and
A Goll, and
H Glossmann
December 1982,
European journal of pharmacology,
