Biomaterial Database

The product of the F sex factor traT surface exclusion gene is a lipoprotein. 1984

N B Perumal, and E G Minkley

The product of the Escherichia coli sex factor F traT gene (TraTp), an outer membrane protein of Mr = 25,000, is covalently modified in vivo by the addition of glycerol and fatty acids. Consistent with this result, and as would be expected for a bacterial lipoprotein, the novel amino acid glycerylcysteine can be detected in purified TraTp. Being a secreted protein, TraTp is made from a signal sequence containing precursor, and glycerol and fatty acids can be detected in both the precursor and mature (processed) species of TraTp. The peptide antibiotic globomycin inhibits the cleavage of the pro-TraTp signal sequence, but not the glycerol and fatty acid modification. Diglyceride modification of the Cys residue at the site of signal sequence cleavage probably precedes and is a prerequisite for processing of the TraTp signal sequence. Thus, TraTp appears to be a typical E. coli lipoprotein, having a pathway for modification and processing that is similar to that of Braun's lipoprotein (the major outer membrane lipoprotein).

UI	MeSH Term	Description	Entries
D008074	Lipoproteins	Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.	Circulating Lipoproteins,Lipoprotein,Lipoproteins, Circulating
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D004262	DNA Restriction Enzymes	Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.	Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005144	F Factor	A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.	Resistance Transfer Factor,Sex Factor F,Sex Factor, Bacterial,Bacterial Sex Factor,Bacterial Sex Factors,F Plasmid,F Plasmids,Factor, Bacterial Sex,Factors, Bacterial Sex,Fertility Factor, Bacterial,Sex Factors, Bacterial,Bacterial Fertility Factor,Bacterial Fertility Factors,F Factors,Factor F, Sex,Factor Fs, Sex,Factor, Bacterial Fertility,Factor, F,Factor, Resistance Transfer,Factors, Bacterial Fertility,Factors, F,Factors, Resistance Transfer,Fertility Factors, Bacterial,Fs, Sex Factor,Plasmid, F,Plasmids, F,Resistance Transfer Factors,Sex Factor Fs,Transfer Factor, Resistance,Transfer Factors, Resistance
D005796	Genes	A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.	Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005798	Genes, Bacterial	The functional hereditary units of BACTERIA.	Bacterial Gene,Bacterial Genes,Gene, Bacterial
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein