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The cloned complementary DNA for coagulation Factor IX (FIX) detects a frequent restriction fragment length polymorphism (RFLP) in human genomic DNAs digested with the restriction endonuclease Taq I. This genetic marker was used, in parallel with coagulation and immunological assays, to follow the segregation of an abnormal FIX gene in a large Hemophilia B family. Among the six potential female carriers, functional assays showed that four had a high probability, and two a low probability of being carriers. Analysis at the DNA level with the cDNA probe was informative in five of the six cases, and in all these five the diagnosis of carrier state was definitively confirmed. This demonstrates the feasibility of using linkage analysis at the DNA level for the genetic screening of Hemophilia B. This method has the advantages over conventional assays of giving a diagnosis of certainty, and of being applicable to early prenatal diagnosis using biopsies of trophoblast villi. At present, the single known polymorphism associated with the FIX gene restricts the application of linkage analysis to informative cases (40%), but findings of additional RFLPs in this region should improve this figure.
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
February 1985,
Blood,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
December 1990,
Sangre,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
March 2013,
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
May 1986,
Blood,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
April 2012,
Acta naturae,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
January 1990,
Zeitschrift fur medizinische Laboratoriumsdiagnostik,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
May 2010,
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
January 1984,
Proceedings of the National Academy of Sciences of the United States of America,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
June 1985,
Nederlands tijdschrift voor geneeskunde,
L Grunebaum, and
J P Cazenave, and
G Camerino, and
C Kloepfer, and
J L Mandel, and
P Tolstoshev, and
M Jaye, and
H De la Salle, and
J P Lecocq
July 1998,
Blood,
