-transparent.png){kind=logo}
Glycocalicin (140 kDa), constituting the main part of glycoprotein Ib (160 kDa), was released from the human platelet membrane by the action of a Ca2+-dependent protease, present in the platelet cytoplasm and liberated during sonication of the platelet suspension. After activation of the protease by Ca2+, the sonicated platelet suspension was subjected to differential centrifugation. The supernatant was applied to a column of wheat germ agglutinin linked to Sepharose 4B; glycocalicin was eluted from the column with 2.5% (w/v) N-acetylglucosamine. Glycocalicin was found to contain 40% carbohydrate by weight, representing N- as well as O-glycosidically linked carbohydrate chains. The O-glycosidic chains were split off by alkaline cleavage in the presence of 3H-labelled NaBH4. The liberated 3H-labelled oligosaccharide-alditols were fractionated on a DEAE-Sephadex A-25 column. The structures of the oligosaccharide-alditols were investigated by 500-MHz 1H-NMR spectroscopy. The major compound was identified as NeuAc alpha(2----3)Ga1 beta(1----3)[NeuAc alpha(2----3)Ga1 beta(1----4)GlcNAc beta(1----6)]GalNAc-ol. Two minor compounds were found to be NeuAc alpha(2----3)Gal beta(1----3)[NeuAc alpha(2----6)]GalNAc-ol and NeuAc alpha(2----3)Gal beta(1----3)GalNAc-ol.
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
January 1987,
Journal of biochemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
February 1984,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
May 1983,
The Journal of biological chemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
December 1984,
European journal of biochemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
September 1994,
The Journal of biological chemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
May 1986,
European journal of biochemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
October 1994,
Glycobiology,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
January 1998,
European journal of biochemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
April 1994,
European journal of biochemistry,
S A Korrel, and
K J Clemetson, and
H Van Halbeek, and
J P Kamerling, and
J J Sixma, and
J F Vliegenthart
December 1993,
Journal of biochemistry,
