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The membrane receptor of human platelets that binds the alpha 1(I)-chain of chick skin collagen was purified to homogeneity by molecular sieve chromatography, affinity chromatography, and preparative gel-electrophoresis. Rabbits that were immunized with the purified receptor emulsified in complete Freund's adjuvant produced antibody, as measured by an enzyme-linked immunosorbent assay. The antiserum was shown to be highly receptor-specific by immunoprecipitation of solubilized platelet membrane protein that had been labeled with (gamma-32P)ATP and protein kinase. Platelet aggregation induced by alpha 1(I) as well as native chick skin collagen was inhibited by both the purified receptor and antibody against the receptor in a dose-dependent fashion. In contrast, neither the receptor nor the antiserum inhibited ADP-induced aggregation. These studies provide the most definitive evidence that alpha 1(I) and native collagen interact specifically with a cell surface receptor on human platelets to induce aggregation.
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
April 1982,
Biochemistry,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
April 1969,
Biochemistry,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
June 2000,
American journal of physiology. Endocrinology and metabolism,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
July 1979,
Biochemical and biophysical research communications,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
August 1969,
Biochemistry,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
February 1974,
The Journal of biological chemistry,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
January 1983,
Connective tissue research,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
December 1969,
Proceedings of the National Academy of Sciences of the United States of America,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
January 1990,
Advances in experimental medicine and biology,
T M Chiang, and
A H Kang, and
J B Dale, and
E H Beachey
October 1983,
The Biochemical journal,
