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Human alcohol dehydrogenase (ADH) is encoded by at least five genes that fall into three classes. The class I ADH genes encode the three closely related alpha, beta, and gamma polypeptides. Molecular genetic analysis of class I ADH genes has been initiated by isolating a cDNA clone from a human adult liver cDNA library. A synthetic oligonucleotide mixture encoding a portion of the beta subunit of ADH was used as an in situ hybridization probe for the cDNA library. One positively hybridizing clone, pADH12, which contained an 1100-base-pair cDNA insert, was subjected to DNA sequence analysis. The sequence indicated that the cDNA encoded information for the carboxyl-terminal 91 amino acids of a class I ADH and a 3' untranslated region of 593 nucleotides. Comparisons with the carboxyl terminus of the human ADH beta subunit indicated that the cDNA encoded the beta polypeptide. This probe may facilitate genetic studies of various human alcohol-related syndromes, as well as enable basic molecular studies on human ADH gene expression.
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
August 1990,
Proceedings of the National Academy of Sciences of the United States of America,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
May 1985,
Proceedings of the National Academy of Sciences of the United States of America,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
August 1994,
Biochemical and biophysical research communications,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
January 1986,
Journal of biochemistry,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
September 1996,
Gene,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
July 1988,
FEBS letters,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
February 1990,
Gene,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
March 1996,
Plant & cell physiology,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
October 1988,
Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
G Duester, and
G W Hatfield, and
R Bühler, and
J Hempel, and
H Jörnvall, and
M Smith
November 1989,
Biochemistry,
