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Freeze-etching of plant cells. 1978

A W Robards

UI MeSH Term Description Entries
D005613 Freeze Etching A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces. Etching, Freeze
D005614 Freeze Fracturing Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica. Fracturing, Freeze,Fracturings, Freeze,Freeze Fracturings
D059828 Plant Cells Basic functional unit of plants. Cell, Plant,Cells, Plant,Plant Cell

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Freeze etching of cells without cryoprotectants.
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[Study of HeLa Cells in culture by freeze etching].
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Ultrastructure of barley aleurone cells as shown by freeze-etching.
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Ultrastructure of Nitrosomonas europaea cells as revealed by freeze-etching.
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