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Three peak-activity fractions of megamodulin were noted in Sephadex G-100 filtration of its crude preparation from baker's yeast (Saccharomyces cerevisiae). Megamodulin thus prepared and its Mg2+-binding complex were shown not only to augment the activity of rabbit brain megamodulin-dependent protein kinase I (M-PK1) but also to interact with arginine-rich histone.
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