Biomaterial Database

Effect of butylated hydroxyanisole on the antifungal activity of amphotericin B. 1983

R F Cosgrove

Monitoring the growth curves of Candida albicans has shown that in the presence of sub-MIC levels of amphotericin B the lag phase is extended. If butylated hydroxyanisole (BHA) is added prior to, or with the amphotericin B the lag phase is extended further. However, if BHA is added after the antibiotic, the growth curve is identical to that produced in the presence of amphotericin B alone. The effect of BHA was found to follow the kinetics of a zero order reaction, and adsorption data for amphotericin B to yeast cells shows that BHA may occupy sites in the cell wall of the organism normally occupied by amphotericin B. It is suggested that if the polyene binding capacity in the cell wall is satisfied by the BHA, then the effective concentration of amphotericin B available to exert its lethal effect on the sterol in the cell membrane is increased.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D002083	Butylated Hydroxyanisole	Mixture of 2- and 3-tert-butyl-4-methoxyphenols that is used as an antioxidant in foods, cosmetics, and pharmaceuticals.	Butylhydroxyanisole,(1,1-Dimethylethyl)-4-methoxyphenol,AMIF-72,BHA,Butyl Methoxyphenol,Embanox,Nipantiox 1-F,Tenox BHA,AMIF 72,AMIF72,Hydroxyanisole, Butylated,Methoxyphenol, Butyl,Nipantiox 1 F,Nipantiox 1F
D002176	Candida albicans	A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).	Candida albicans var. stellatoidea,Candida stellatoidea,Dematium albicans,Monilia albicans,Myceloblastanon albicans,Mycotorula albicans,Parasaccharomyces albicans,Procandida albicans,Procandida stellatoidea,Saccharomyces albicans,Syringospora albicans
D002473	Cell Wall	The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.	Cell Walls,Wall, Cell,Walls, Cell
D000666	Amphotericin B	Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela.	Amphocil,Amphotericin,Amphotericin B Cholesterol Dispersion,Amphotericin B Colloidal Dispersion,Fungizone
D000840	Anisoles	A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.	Methylphenyl Ethers,Ethers, Methylphenyl
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor