Biomaterial Database

A polyamine-sensitive mutant of Aspergillus nidulans. 1983

D H Spathas, and A J Clutterbuck, and J A Pateman

A mutation designated spsA1 has been induced in the putrescine (puA2) auxotroph of Aspergillus nidulans which enables this mutant to grow on low concentrations of spermidine in place of putrescine. In addition, the spsA1 mutant, irrespective of putrescine requirement, is abnormally sensitive to high concentrations of spermidine, spermine or the polyamine analogue methylglyoxal bis(guanylhydrazone). When spsA1 strains are grown on medium containing spermidine, uptake of the polyamine continues at a high level for a longer period than in the wild-type and leads to a doubled intracellular spermidine pool. A similar increase in the intracellular spermine pool results from growth on spermine.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D009584	Nitrogen	An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D011073	Polyamines	Amine compounds that consist of carbon chains or rings containing two or more primary amino groups.	Polyamine
D011700	Putrescine	A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine.	1,4-Butanediamine,1,4-Diaminobutane,Tetramethylenediamine,1,4 Butanediamine,1,4 Diaminobutane
D001233	Aspergillus nidulans	A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.	Aspergillus nidulellus,Emericella nidulans
D013095	Spermidine	A polyamine formed from putrescine. It is found in almost all tissues in association with nucleic acids. It is found as a cation at all pH values, and is thought to help stabilize some membranes and nucleic acid structures. It is a precursor of spermine.
D013096	Spermine	A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.