Biomaterial Database

Regulation of expression of novel mandelate dehydrogenases in mutants of Acinetobacter calcoaceticus. 1983

C A Hills, and C A Fewson

Wild-type strains of Acinetobacter calcoaceticus able to grow on only L(+)- or D(-)-mandelate gave rise to mutants that could grow on the other isomer of mandelate. Each mutant contained an additional mandelate dehydrogenase which was not expressed in the parent strain. The novel enzymes were shown to be controlled co-ordinately with the pre-existing enzymes for the conversion of mandelate into benzoate when induced with phenylglyoxylate, gratuitously induced with thiophenoxyacetate, subjected to anti-induction by 2-phenylpropionate or catabolite-repressed by succinate. Mutants which had been selected on the basis of possession of a constitutive phenylglyoxylate decarboxylase also constitutively expressed both the original and the novel mandelate dehydrogenases.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D002262	Carboxy-Lyases	Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.	Carboxy-Lyase,Decarboxylase,Decarboxylases,Carboxy Lyase,Carboxy Lyases
D004790	Enzyme Induction	An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.	Induction, Enzyme
D004794	Enzyme Repression	The interference in synthesis of an enzyme due to the elevated level of an effector substance, usually a metabolite, whose presence would cause depression of the gene responsible for enzyme synthesis.	Repression, Enzyme
D005786	Gene Expression Regulation	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.	Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000150	Acinetobacter	A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.	Herellea,Mima
D000429	Alcohol Oxidoreductases	A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).	Carbonyl Reductase,Ketone Reductase,Carbonyl Reductases,Ketone Reductases,Oxidoreductases, Alcohol,Reductase, Carbonyl,Reductase, Ketone,Reductases, Carbonyl,Reductases, Ketone