Timing of oxytocin release may influence milk ejection. Oxytocin is higher in cows after manual premilking stimulation than in cows receiving no stimulation. Premilking stimulation offers no advantages to milk production in short trials but results in shorter times of machine on and higher average rate of milk flow. Enhanced mechanical stimulation does not seem as effective as manual stimulation in short trials. Oxytocin release is multiphasic, and contents in blood decline during milking. Maximal concentrations during milking decrease across lactation. Nonlactating cows also release oxytocin upon udder stimulation and conditioning. Virgin heifers do not respond in the same way to udder stimulation as lactating cows, thus suggesting that sensitivity of the milk ejection reflex depends upon the physiological state of the animal. Cows probably release about one-third of their stores of oxytocin at milking, and it appears that little hormone is necessary for effect at the udder. Basal concentrations of oxytocin decrease from early to midlactation and increase from mid to late lactation and from late lactation to involution. Half-lives, metabolic clearance rates, and entry rates for oxytocin were measured in cattle in various physiological states. Data from these measurements are discussed.