Biomaterial Database

Oxidation of carbohydrates and palmitate by intact cultured neonatal rat heart cells. 1984

P D Ross, and R L McCarl

The rates of uptake and oxidation of glucose, lactate, pyruvate, and palmitate were measured for "mixed" cultures of rat heart cells that exhibit a myocyte-to-fibroblast ratio similar to that observed in vivo. Glucose uptake and conversion to lactate were also measured using enriched cultures of myocytes and fibroblasts. The metabolism of mixed cultures, which contain 70-80% myocytes, closely resembles that of enriched myocyte cultures. The energy production and substrate oxidation rates of cultured neonatal heart cells, adult myocytes, and perfused hearts are compared. It appears that the energy requirements of cultured heart cells are much lower than that of whole tissue. The results suggest that the metabolism of cultured heart cells may be basically the same as those in vivo but appears to be different because of reduced energy requirements in culture.

UI	MeSH Term	Description	Entries
D007773	Lactates	Salts or esters of LACTIC ACID containing the general formula CH3CHOHCOOR.
D009206	Myocardium	The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.	Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D010168	Palmitates	Salts and esters of the 16-carbon saturated monocarboxylic acid--palmitic acid.	Hexadecanoates,Palmitate
D010169	Palmitic Acids	A group of 16-carbon fatty acids that contain no double bonds.	Acids, Palmitic
D011773	Pyruvates	Derivatives of PYRUVIC ACID, including its salts and esters.
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003584	Cytological Techniques	Methods used to study CELLS.	Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006003	Glycogen