-transparent.png){kind=logo}
We compared the survival of a laboratory strain of Chlamydia trachomatis serovar L-2 in different media and at different temperatures (room temperature, 4 degrees C, and -70 degrees C). At these temperatures the best storage medium was 2SP (0.2 mol/l sucrose in 0.02 mol/l phosphate buffer supplemented with 10% fetal calf serum). We used material obtained from patients to study the sensitivity of the culture method as a function of sample storage time and temperature. Compared with results on direct inoculation, material stored in 2SP for 48 hours gave 11% fewer positive cultures at 4 degrees C and 14% fewer at room temperature. Of samples which gave negative results on direct inoculation, 4% were positive after storage at 4 degrees C for 48 hours and 2% after storage at -70 degrees C for a week. As expected, the number of inclusion forming units in the original material proved to be important for the percentage of positive cultures among the stored samples.
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
January 1983,
Acta microbiologica Hungarica,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
December 1991,
The Central African journal of medicine,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
January 1982,
Pediatric infectious disease,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
September 1986,
The Western journal of medicine,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
March 1989,
Papua and New Guinea medical journal,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
January 2019,
Frontiers in cell and developmental biology,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
January 1981,
Czechoslovak medicine,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
July 2005,
Nihon rinsho. Japanese journal of clinical medicine,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
June 1985,
Genitourinary medicine,
K H Tjiam, and
B Y van Heijst, and
J C de Roo, and
A de Beer, and
T van Joost, and
M F Michel, and
E Stolz
May 2007,
Journal of clinical microbiology,
