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Variant endothelial cells were cultured from the healthy carotid arteries of a 19-year-old woman who was killed accidentally. The cells were grown and subcultured in Medium 199 supplemented with 20% heat-inactivated fetal calf serum. The cells are still viable after 20 passages. They were recognized as variant endothelial cells by their morphology which included ring formation, and by the existence of factor VIII-related surface antigen on the cell membrane. The cultured endothelial cells produced prostacyclin when the cells were incubated with arachidonic acid in smaller amounts than does typical endothelium, but far in excess of that produced by vascular smooth muscle cells.
O Tokunaga, and
M Morimatsu, and
T Nakashima
October 1999,
Fukuoka igaku zasshi = Hukuoka acta medica,
O Tokunaga, and
M Morimatsu, and
T Nakashima
December 1985,
Atherosclerosis,
O Tokunaga, and
M Morimatsu, and
T Nakashima
January 1980,
Methods in cell biology,
O Tokunaga, and
M Morimatsu, and
T Nakashima
April 1977,
Clinical and experimental immunology,
O Tokunaga, and
M Morimatsu, and
T Nakashima
October 2007,
Arteriosclerosis, thrombosis, and vascular biology,
O Tokunaga, and
M Morimatsu, and
T Nakashima
October 1994,
Thrombosis research,
O Tokunaga, and
M Morimatsu, and
T Nakashima
April 1995,
Archives of ophthalmology (Chicago, Ill. : 1960),
O Tokunaga, and
M Morimatsu, and
T Nakashima
January 2007,
Microvascular research,
O Tokunaga, and
M Morimatsu, and
T Nakashima
January 1993,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
O Tokunaga, and
M Morimatsu, and
T Nakashima
January 1974,
Tissue antigens,
