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A conjugate of the monoclonal antibody WT1 and ricin A-chain was studied for its suitability for purging marrow of leukemic T cells for autologous transplantation in T cell acute lymphocytic leukemia (T-ALL). The conjugate was powerfully cytotoxic to the human T-ALL cell line, GH1, which expresses the WT1 antigen at a high density. Treatment of the cells with the conjugate at 10(-11) M reduced their rate of protein synthesis by 50%, and the inclusion of 6 mM ammonium chloride in the cultures enhanced the potency of cytotoxic effect by 10-100-fold. Clonogenic assays indicated that less than 0.1% of GH1 cells survived 3-hr exposure to the conjugate in ammonium chloride. WT1 alone did not react with multipotent (CFU-GEMM) hematopoietic progenitors in normal human bone marrow, as measured by fluorescence-activated cell sorting. Under conditions giving maximal killing of GH1 cells, there was no toxicity to multipotential progenitors in normal human marrow.
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
August 1989,
Blood,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
January 1987,
Haematology and blood transfusion,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
February 1989,
British journal of haematology,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
January 1994,
International journal of immunopharmacology,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
January 1987,
Blood,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
June 1985,
Blood,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
July 1984,
The Journal of experimental medicine,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
December 1991,
Cancer research,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
June 1986,
Blood,
C D Myers, and
P E Thorpe, and
W C Ross, and
A J Cumber, and
F E Katz, and
W Tax, and
M F Greaves
July 2003,
Leukemia,
