BIOMAT Database Logo BIOMAT Database Logo

Binding of cytoskeletal proteins by monoclonal anti-DNA lupus autoantibodies. 1984

J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz

Monoclonal anti-DNA antibodies produced by hybridomas derived from MRL-lpr/lpr mice and human lupus patients were found to bind to the cytoskeleton of mink lung cells. When tested by indirect immunofluorescence, 17/29 human monoclonal anti-DNA antibodies reacted with the cytoskeleton; 4 of the 29 also produce antinuclear reactions with epithelial cells. The cytoskeletal staining was not inhibited by prior treatment of the cells with DNase, but it was completely blocked by prior incubation of the monoclonal antibodies with DNA and other nucleic acids. The ability of the polynucleotides to inhibit the cytoskeletal staining corresponded to their ability to bind to the antibodies in competitive immunoassays. An (Fab')2 preparation of a monoclonal antibody bound to the cytoskeleton as well as the whole immunoglobulin. The effect of colcemid on the staining pattern, the blocking effect of a monoclonal antivimentin antibody, and results with nitrocellulose blots of cellular proteins indicated that the cytoskeletal protein to which the antibodies bound was vimentin.

UI MeSH Term Description Entries
D007381 Intermediate Filament Proteins Filaments 7-11 nm in diameter found in the cytoplasm of all cells. Many specific proteins belong to this group, e.g., desmin, vimentin, prekeratin, decamin, skeletin, neurofilin, neurofilament protein, and glial fibrillary acid protein. Fibroblast Intermediate Filament Proteins,Filament Proteins, Intermediate,Proteins, Intermediate Filament
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008180 Lupus Erythematosus, Systemic A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Libman-Sacks Disease,Lupus Erythematosus Disseminatus,Systemic Lupus Erythematosus,Disease, Libman-Sacks,Libman Sacks Disease
D008817 Mice, Mutant Strains Mice bearing mutant genes which are phenotypically expressed in the animals. Mouse, Mutant Strain,Mutant Mouse Strain,Mutant Strain of Mouse,Mutant Strains of Mice,Mice Mutant Strain,Mice Mutant Strains,Mouse Mutant Strain,Mouse Mutant Strains,Mouse Strain, Mutant,Mouse Strains, Mutant,Mutant Mouse Strains,Mutant Strain Mouse,Mutant Strains Mice,Strain Mouse, Mutant,Strain, Mutant Mouse,Strains Mice, Mutant,Strains, Mutant Mouse
D008907 Mink Carnivores of genera Mustela and Neovison of the family MUSTELIDAE. The European mink has white upper and lower lips while the American mink lacks white upper lip. American Mink,European Mink,Mustela lutreola,Mustela macrodon,Mustela vison,Neovison vison,Sea Mink,Mink, American,Mink, European,Mink, Sea,Minks,Minks, Sea,Sea Minks,vison, Neovison
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Anti-DNA autoantibodies: the other DNA-binding proteins.
March 1997, Bioorganic & medicinal chemistry,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies.
June 1985, The Journal of experimental medicine,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
IgG human monoclonal anti-DNA autoantibodies from patients with systemic lupus erythematosus.
September 1991, Clinical and experimental immunology,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Hybridoma lupus autoantibodies can bind major cytoskeletal filaments in the absence of DNA-binding activity.
July 1988, Arthritis and rheumatism,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Platelet binding properties of monoclonal lupus autoantibodies produced by human hybridomas.
December 1985, Blood,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Binding of monoclonal anti-native DNA autoantibodies to DNA of varying size and conformation.
December 1985, Molecular immunology,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Monoclonal lupus autoantibodies.
March 1983, Immunology today,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Monoclonal lupus autoantibodies.
July 1982, Arthritis and rheumatism,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Repertoire cloning of lupus anti-DNA autoantibodies.
December 1996, The Journal of clinical investigation,
J André-Schwartz, and S K Datta, and Y Shoenfeld, and D A Isenberg, and B D Stollar, and R S Schwartz
Anti-DNA autoantibodies and systemic lupus erythematosus.
August 1999, Pharmacology & therapeutics,
Copied contents to your clipboard!