-transparent.png){kind=logo}
The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3'-ends and blocked 5'-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5'termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.
Related Publications
Y Kikuchi, and
K Hirai, and
F Hishinuma
August 1984,
Journal of bacteriology,
Y Kikuchi, and
K Hirai, and
F Hishinuma
February 1987,
Nucleic acids research,
Y Kikuchi, and
K Hirai, and
F Hishinuma
June 1985,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Y Kikuchi, and
K Hirai, and
F Hishinuma
September 1986,
Yeast (Chichester, England),
Y Kikuchi, and
K Hirai, and
F Hishinuma
December 1988,
Molecular & general genetics : MGG,
Y Kikuchi, and
K Hirai, and
F Hishinuma
July 1981,
Journal of bacteriology,
Y Kikuchi, and
K Hirai, and
F Hishinuma
April 1987,
Biochemical and biophysical research communications,
Y Kikuchi, and
K Hirai, and
F Hishinuma
March 1994,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Y Kikuchi, and
K Hirai, and
F Hishinuma
October 1984,
Nucleic acids research,
Y Kikuchi, and
K Hirai, and
F Hishinuma
August 1988,
Nucleic acids research,
