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A practical enzyme immunoassay (EIA) has been developed for the measurement of anti-acetylcholine receptor (AChR) antibodies in sera from patients with myasthenia gravis (MG). This system is based on the double antibody technique, using denervated rat muscle AChR labeled with horseradish peroxidase-linked alpha-bungarotoxin (HRP-alpha BGT). This method has the following advantages compared to conventional radioimmunoassay (RIA): (1) HRP-alpha BGT is more stable than [125I]alpha BGT and can be used for at least one year without any loss of the binding activity to AChR and enzymatic activity, (2) the procedure avoids the use of radioactive isotopes, and (3) the equipment for our EIA is more economical than that for RIA.
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
May 1981,
Italian journal of neurological sciences,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
October 1984,
Journal of immunological methods,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
January 2009,
Frontiers of neurology and neuroscience,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
May 1984,
Clinical immunology and immunopathology,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
January 1988,
Journal of receptor research,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
December 1980,
Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
October 1975,
The New England journal of medicine,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
April 1983,
Harefuah,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
January 1981,
Muscle & nerve,
S Furukawa, and
S Akazawa, and
Y Furukawa, and
I Kamo, and
E Satoyoshi, and
K Hayashi
January 1981,
Journal of neurology,
