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The production of antisera to specific proteins using as immunogens short, synthetic oligopeptides corresponding in sequence to regions of the proteins is analysed. Of 103 oligopeptides used for this purpose and reported in the literature before the end of 1983 all those corresponding to N or C terminal sequences produced antisera reacting with the complete protein. Of 69 oligopeptides corresponding to internal sequences only 71% were successfully used to prepare antisera. An analysis of these 69 oligopeptides showed that peptides of less than 10 amino acids were unlikely to produce useful antisera and that the more hydrophilic peptides were marginally more useful than those less hydrophilic.
J W Palfreyman, and
T C Aitcheson, and
P Taylor
April 1992,
BioTechniques,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
February 1977,
Immunochemistry,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
June 1972,
Biology of reproduction,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
May 1984,
The Journal of general virology,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
January 1986,
Current topics in microbiology and immunology,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
January 1983,
Annual review of microbiology,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
November 1983,
Proceedings of the National Academy of Sciences of the United States of America,
J W Palfreyman, and
T C Aitcheson, and
P Taylor
January 1988,
Methods in molecular biology (Clifton, N.J.),
J W Palfreyman, and
T C Aitcheson, and
P Taylor
January 1994,
Methods in molecular biology (Clifton, N.J.),
J W Palfreyman, and
T C Aitcheson, and
P Taylor
June 1993,
Gene,
