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8 S RNA of Novikoff hepatoma was characterized by fingerprinting, sequencing gels, and by hybridization to rat ribosomal DNA clones. The data obtained show that 8 S RNA is 273 or 274 nucleotides long; ribosomal 5.8 S RNA is its 5'-terminal 156 nucleotides. All the post-transcriptional modifications found in 5.8 S rRNA were also found in 8 S RNA; no other modifications were found. The 3'-terminal 118 nucleotides were consistent with the adjoining internal transcribed spacer sequence in rDNA (Subrahmanyam, C. S., Cassidy, B., Busch, H., and Rothblum, L. (1982) Nucleic Acids Res. 10, 3667-3680). Based on its nucleolar localization, the finding that all the 8 S RNA is hydrogen-bonded to preribosomal RNA and its consistency in sequence to the cloned rat ribosomal DNA sequence, it appears that 8 S RNA is a relatively stable intermediate in the formation of 5.8 S rRNA from 45 S pre-rRNA. This stable intermediate RNA may be a useful substrate for studies on rRNA processing and for studies on eukaryotic rRNA-processing enzyme(s).
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
September 1974,
FEBS letters,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
January 1975,
Biochemistry,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
January 1974,
Physiological chemistry and physics,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
May 1972,
The Journal of biological chemistry,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
June 1977,
The Journal of biological chemistry,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
December 1976,
FEBS letters,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
October 1985,
The Journal of biological chemistry,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
July 1977,
Nucleic acids research,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
January 1969,
Journal of molecular biology,
R Reddy, and
L I Rothblum, and
C S Subrahmanyam, and
M H Liu, and
D Henning, and
B Cassidy, and
H Busch
October 1972,
FEBS letters,
