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The addition of purified factor IXa and phospholipid to factor VIII concentrate protected the VIII:C from inactivation by human antibodies. This protective effect was shown to be due largely to the phospholipid. Addition of phospholipid alone gave substantial protection against even high-titer antibodies, as shown by measurements of thrombin generation and VIII:C assays. Increasing concentrations of phospholipid led to significant reductions in the amount of VIII C:Ag detected by an IRMA method, up to 70% of the original VIII C:Ag being "lost" at the highest concentration of phospholipid. These results indicate that phospholipid binding plays an important part in the procoagulant activity of factor VIII and that human antibodies to VIII:C are directed largely at the phospholipid binding site. The addition of phospholipid to factor VIII concentrates could have important clinical applications in the treatment of hemophiliacs with antibodies to factor VIII.
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
January 1986,
Annals of clinical and laboratory science,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
January 1990,
The Journal of biological chemistry,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
June 1989,
The Journal of clinical investigation,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
November 1984,
Biochemical and biophysical research communications,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
July 1998,
Blood,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
November 1982,
Blood,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
June 2003,
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
June 1995,
The Journal of biological chemistry,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
February 1980,
Thrombosis and haemostasis,
T W Barrowcliffe, and
G Kemball-Cook, and
E Gray
August 1992,
The Journal of biological chemistry,
