-transparent.png){kind=logo}
We have purified S-100 protein from bovine brain using Ca2+-dependent affinity chromatography on N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7)-Sepharose (Endo, T., Tanaka, T., Isobe, T., Kasai, H., Okuyama, T., and Hidaka, H. (1981) J. Biol. Chem. 256, 12485-12489). By essentially the same procedure, W-7-Sepharose binding protein has been purified to apparent homogeneity from bovine abdominal adipose tissue. Electrophoretically, the purified protein from adipose tissue co-migrated with brain S-100b protein both in the presence and absence of sodium dodecyl sulfate and the protein was indistinguishable from brain S-100b region in terms of amino acid composition, two-dimensional tryptic peptide mapping and reactivity with anti-brain S-100b serum. Immunohistochemical analysis confirmed the existence of S-100b protein in the adipose cell where the protein seems to be located in both the nucleus and cytoplasm. Thus, the results indicate that the adipose cells contain the protein possibly identical with brain S-100b protein. In addition, the contents of S-100b protein in various rat tissues were measured by enzyme immunoassay method using the anti-bovine brain S-100b serum. Significant amounts of S-100b protein were found not only in the adipose tissue but also in the peripheral tissue such as trachea and skin. These observations suggest that S-100b protein should no longer be considered as a protein specific to nervous tissues.
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
January 2013,
Frontiers in neurology,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
January 1983,
The International journal of biochemistry,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
December 1982,
The Biochemical journal,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
November 1982,
Biochimica et biophysica acta,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
April 2001,
Clinical chemistry and laboratory medicine,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
January 2002,
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
April 1974,
The Journal of biological chemistry,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
January 1982,
The International journal of biochemistry,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
April 2000,
Neurosurgery,
H Hidaka, and
T Endo, and
S Kawamoto, and
E Yamada, and
H Umekawa, and
K Tanabe, and
K Hara
September 1999,
Neurosurgery,
