BIOMAT Database Logo BIOMAT Database Logo

Radiometric microbiological assay of vitamin B6: assay simplification and sensitivity study. 1983

T R Guilarte

Modification of a previously developed radiometric microbiological assay for vitamin B6 reduces assay complexity and time. Reduction of enzymatic treatment from 24 to 3 h essentially eliminates one day's time for the analysis of plasma samples. Use of lyophilized Kloeckera brevis cultures eliminates routine subculturing of the test organism, with no significant effect on test results. Modifications in test vial size and total volume in test vials have increased assay sensitivity to a level of 0.25 ng pyridoxine (PN), pyridoxal (PL), or pyridoxamine (PM) per vial level and decreased the amount of medium and labeled substrate (i.e., L-[1-14C]-valine), thus reducing assay cost.

UI MeSH Term Description Entries
D011736 Pyridoxine The 4-methanol form of VITAMIN B 6 which is converted to PYRIDOXAL PHOSPHATE which is a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. Although pyridoxine and Vitamin B 6 are still frequently used as synonyms, especially by medical researchers, this practice is erroneous and sometimes misleading (EE Snell; Ann NY Acad Sci, vol 585 pg 1, 1990). Pyridoxin,Pyridoxine Hydrochloride,Pyridoxol,Pyridoxol Hydrochloride,Rodex
D002245 Carbon Dioxide A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals. Carbonic Anhydride,Anhydride, Carbonic,Dioxide, Carbon
D002250 Carbon Radioisotopes Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes. Radioisotopes, Carbon
D003904 Mitosporic Fungi A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group. Deuteromycetes,Deuteromycota,Fungi imperfecti,Fungi, Mitosporic,Hyphomycetes,Deuteromycete,Deuteromycotas,Fungi imperfectus,Fungus, Mitosporic,Hyphomycete,Mitosporic Fungus,imperfectus, Fungi
D005504 Food Analysis Measurement and evaluation of the components of substances to be taken as FOOD. Analysis, Food,Analyses, Food,Food Analyses
D001681 Biological Assay A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc. Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D014633 Valine A branched-chain essential amino acid that has stimulant activity. It promotes muscle growth and tissue repair. It is a precursor in the penicillin biosynthetic pathway. L-Valine,L Valine

Related Publications

T R Guilarte
Aspects of microbiological methods for vitamin assay; assay of vitamin B6.
January 1957, Svensk farmaceutisk tidskrift,
T R Guilarte
The microbiological assay of vitamin B6 in urine.
March 1962, Tubercle,
T R Guilarte
The microbiological assay of the vitamin B6 complex (pyridoxine, pyridoxal and pyridoxamine) with Kloeckera brevis.
April 1971, The Analyst,
T R Guilarte
Microbiological assay of vitamin B6 by thin-layer cup-plate method with Saccharomyces carlsbergensis.
June 1972, The Journal of vitaminology,
T R Guilarte
[DEGRADATION OF VITAMIN B6 VIA A MICROBIOLOGICAL PATHWAY].
August 1963, Archives internationales de physiologie et de biochimie,
T R Guilarte
[ON THE MICROBIOLOGICAL METHOD OF DETERMINING VITAMIN B6].
January 1964, Laboratornoe delo,
T R Guilarte
Biochemical mutants of microorganisms in vitamin assay; assay of vitamin B6.
May 1955, Svensk farmaceutisk tidskrift,
T R Guilarte
Microbiological assay of vitamin B12.
January 1966, Methods of biochemical analysis,
T R Guilarte
Microbiological assay of vitamin B12.
January 1954, Methods of biochemical analysis,
T R Guilarte
Microbiological method of vitamin assay.
January 1983, International journal for vitamin and nutrition research. Supplement = Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Supplement,
Copied contents to your clipboard!