-transparent.png){kind=logo}
The hydroxylation of n-hexane by rat liver microsomes was studied and the contribution of different molecular species of cytochrome P-450 to the hydroxylation reaction was examined. In the case of untreated rats, the products of NADPH-dependent n-hexane hydroxylation were 1-, 2-, and 3-hexanols, and the major one was 2-hexanol. Phenobarbital (PB) treatment of animals resulted in a significant increase of the hydroxylation activity. The formation of 2- and 3-hexanols was much more significantly increased than that of 1-hexanol. On the other hand, 3-methylcholanthrene (MC) treatment stimulated the formation of 3-hexanol and the formation of the other two isomeric alcohols was rather decreased. These observations suggested the position specificities of the PB-inducible form (P-450(PB)) and MC-inducible form (P-450(MC)) of cytochrome P-450 in the hydroxylation of n-hexane. Inhibition experiments using antibodies specific to P-450(PB) and P-450(MC) also indicated that P-450(PB) was more active in the hydroxylation at the 2-position whereas P-450(MC) was more specific for the 3-position. NADPH-dependent n-hexane hydroxylation systems were reconstituted by the use of purified NADPH-cytochrome P-450 reductase and P-450(PB) or P-450(MC), and the activities of the reconstituted systems supported the proposed position specificities of these two forms of cytochrome P-450 in n-hexane hydroxylation.
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
January 1990,
Drug metabolism and disposition: the biological fate of chemicals,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
November 1986,
Biochemical pharmacology,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
July 1990,
Biokhimiia (Moscow, Russia),
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
January 1993,
Drug metabolism and disposition: the biological fate of chemicals,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
October 1984,
The Journal of pharmacology and experimental therapeutics,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
April 1986,
Chemico-biological interactions,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
July 1974,
Biochimica et biophysica acta,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
June 1972,
La Nouvelle presse medicale,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
August 1989,
Biulleten' eksperimental'noi biologii i meditsiny,
K Morohashi, and
H Sadano, and
Y Okada, and
T Omura
February 1975,
FEBS letters,
