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Ciliated cortices isolated from rabbit trachea or oviduct can be reactivated with MgATP (2.4 mM, approx). We analyzed the effects of varying concentrations (0.1-100 mM) of Ca2+ and Mn2+ on the number of cortices which reactivated, as a percentage of the controls. Although reactivation can be inhibited at approximately 10(-2) M cation, we believe that this inhibition might be the result of decreasing MgATP due to competition by Ca2+ and Mn2+ for ATP cation binding sites, rather than cation interactions with the axoneme proper. In addition, whole isolated ciliated cells were obtained from trachea or oviduct by a simple, one step pronase digestion. These cells were incubated with the calcium ionophore A23187 in the presence of 2 mM Ca2+. Inhibition of ciliary activity in 50% of the cells did not occur until about 60 min of incubation. It is believed that what we have observed is cell toxicity rather than a direct effect on the cilia.
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