Determination of the sequence which spans the beginning of the insertion region in Anacystis nidulans flavodoxin. 1983

G E Tarr

Anacystis nidulans flavodoxin is one of the long chain flavodoxins (Mayhew & Ludwig, 1975). Comparisons of its structure with the structures of shorter chain species (main text: Ludwig et al., 1982) show that in A. nidulans flavodoxin most of the extra residues occupy a region adjoining the third helix and the fifth strand of parallel sheet. The sequences of peptides isolated after cyanogen bromide cleavage and after digestion with Staphylococcus aureus protease, reported here, fit into the electron density map of A. nidulans flavodoxin, starting near the middle of the third helix, and verify that the major insertion interrupts the fifth strand of parallel sheet.

UI MeSH Term Description Entries
D005418 Flavodoxin A low-molecular-weight (16,000) iron-free flavoprotein containing one molecule of flavin mononucleotide (FMN) and isolated from bacteria grown on an iron-deficient medium. It can replace ferredoxin in all the electron-transfer functions in which the latter is known to serve in bacterial cells.
D005420 Flavoproteins Flavoprotein
D000458 Cyanobacteria A phylum of oxygenic photosynthetic bacteria comprised of unicellular to multicellular bacteria possessing CHLOROPHYLL a and carrying out oxygenic PHOTOSYNTHESIS. Cyanobacteria are the only known organisms capable of fixing both CARBON DIOXIDE (in the presence of light) and NITROGEN. Cell morphology can include nitrogen-fixing heterocysts and/or resting cells called akinetes. Formerly called blue-green algae, cyanobacteria were traditionally treated as ALGAE. Algae, Blue-Green,Blue-Green Bacteria,Cyanophyceae,Algae, Blue Green,Bacteria, Blue Green,Bacteria, Blue-Green,Blue Green Algae,Blue Green Bacteria,Blue-Green Algae
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
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