-transparent.png){kind=logo}
The distance between the heme iron and the N-terminus of cytochrome P-450 LM2 was determined by fluorescence energy transfer measurements. Fluorescein isothiocyanate which was covalently bound to the N-terminal methionine was used as donor chromophor. The Ro value between fluorescein isothiocyanate and the heme was calculated to be 3.98 nm. The distance between the nitrogen of the N-terminal methionine and the heme was estimated with 2.84 +/- 0.23 nm excluding most likely the N-terminal amino acid of cytochrome P-450 LM2 to participate in the electron transfer to the heme iron. A cytochrome P-450 LM2 membrane model is proposed.
Related Publications
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
September 1989,
Biochemical and biophysical research communications,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
January 1988,
Biomedica biochimica acta,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
February 1988,
Biochemical and biophysical research communications,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
January 1986,
Biochemical and biophysical research communications,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
April 1989,
The Journal of biological chemistry,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
January 1982,
Acta biologica et medica Germanica,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
March 1986,
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
July 1986,
European journal of biochemistry,
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
September 1989,
Biokhimiia (Moscow, Russia),
W Schwarze, and
R Bernhardt, and
G R Jänig, and
K Ruckpaul
January 1988,
The Journal of biological chemistry,
