A comparison of microaerobic systems for the culture of Campylobacter jejuni and Campylobacter coli. 1983

F J Bolton, and D Coates

The relative ability of two commercial gas generating envelopes, the evacuation-replacement technique, and a candle jar, to produce a satisfactory microaerobic atmosphere for the culture of 45 Campylobacter strains on non-selective medium and five selective media (Skirrow's, modified Butzler's Blaser's, Campy-BAP and Preston medium) was investigated quantitatively. A candle jar, and modified Butzler's medium proved to be of limited use. The ability of four commercial gas generating envelopes to produce a satisfactory microaerobic atmosphere in four anaerobic jars of different volume was investigated using five reference Campylobacter strains. Not all of the combinations worked. The oxygen and carbon dioxide concentrations produced in the microaerobic systems studied were measured with gas analysers. The evacuation-replacement technique produced far less variable concentrations of oxygen and carbon dioxide than did the envelopes.

UI MeSH Term Description Entries
D010100 Oxygen An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration. Dioxygen,Oxygen-16,Oxygen 16
D002167 Campylobacter A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.
D002245 Carbon Dioxide A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals. Carbonic Anhydride,Anhydride, Carbonic,Dioxide, Carbon
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D000332 Aerobiosis Life or metabolic reactions occurring in an environment containing oxygen. Aerobioses

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