-transparent.png){kind=logo}
Interferon (IFN)-gamma was produced with a high yield in cultures of human peripheral mononuclear cells by combined stimulation with OK-432 and staphylococcal enterotoxin B. Human mononuclear cells cultured in serum-free medium produced several times as much IFN as those in RPMI-1640 medium containing 10% fetal bovine serum. A synergistic effect of OK-432 and staphylococcal enterotoxin B on the production of IFN-gamma was demonstrated. Ultrogel AcA54 column chromatography of crude IFN showed a single peak with an apparent molecular weight of 43,000. Our production system for human IFN-gamma offers a feasible approach to preparation of large quantities of purified IFN-gamma for structure studies, antibody production, and clinical applications.
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
January 1973,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
May 1979,
In vitro,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
March 1982,
Experimental cell research,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
November 1969,
Applied microbiology,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
April 1985,
Journal of immunological methods,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
January 1984,
Journal of interferon research,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
September 1984,
Molecular immunology,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
January 1980,
Journal of immunological methods,
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
September 1981,
Biomedicine / [publiee pour l'A.A.I.C.I.G.],
M Tsujimoto, and
T Tarutani, and
K Ogawa, and
K Akashi, and
Y Okubo, and
N Higashi
July 1988,
Clinical allergy,
