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Rabbit antisera were prepared against three highly purified enzymes from bovine spleen: proteinase I (cathepsin L), proteinase II (cathepsin H), and cathepsin B. The Ouchterlony double diffusion test shows that each antiserum specifically reacts with the corresponding antigen and does not cross react with other proteinases. These data provide evidence that the three proteinases are distinct with respect to their antigenic properties. Using specific antisera, the identity of two preparations of proteinase I isolated by different methods was demonstrated. Analysis of the fractions obtained in the course of isolation procedure revealed a component reacting with antisera against proteinase I. It had a greater molecular mass than proteinase I (30 000-40 000), was richer in antigenic respect and had a lower proteolytic activity as compared with proteinase I. The effect of various inhibitors and denaturation conditions on antigenic properties of proteinases was also studied.
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
January 1981,
Acta biologica et medica Germanica,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
July 1984,
Japanese journal of pharmacology,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
December 1984,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
July 1991,
FEBS letters,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
December 1989,
The Biochemical journal,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
February 1992,
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
March 1985,
FEBS letters,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
January 1991,
Biomedica biochimica acta,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
January 1982,
The Biochemical journal,
L A Lokshina, and
I A Tarkhanova, and
O N Lubkova, and
N V Golubeva, and
T A Gureeva
July 1999,
Cancer biochemistry biophysics,
