-transparent.png){kind=logo}
The effect of 6-hydroxydopamine on the catecholamine content and cell morphology of bovine adrenal chromaffin cells in culture was investigated. 6-Hydroxydopamine markedly reduced the catecholamine content of the cultured chromaffin cells after 6 and 24 h exposure. The effect was dose-related, with half-maximal depletion occurring at 2.4 X 10(-5) M. Cells exposed to 6-hydroxydopamine for 3 h and then to normal medium without the drug for 3 h more showed the same degree of toxicity as cells exposed to the drug for the entire 6 h. Ascorbate at high concentrations also exhibited toxicity toward chromaffin cells between 6 and 24 h of exposure. 6-Hydroxydopamine produced marked changes in cell morphology. At 1 h the cells appeared normal, at 3 h the processes were markedly shortened, and at 6 h they were completely retracted. On exposure for 24 h there were gross morphological changes and most cells were detached and free-floating in the medium. The toxicity of 6-hydroxydopamine in bovine adrenal chromaffin cells is discussed.
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
January 1999,
Life sciences,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
January 1987,
Synapse (New York, N.Y.),
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
April 1983,
Journal of neuroscience methods,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
April 1990,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
January 2007,
Nature protocols,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
April 1991,
Neurochemical research,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
February 1997,
Neuroscience letters,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
December 1999,
British journal of pharmacology,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
January 1983,
Biochemical pharmacology,
S M Kirpekar, and
J Nobiletti, and
J M Trifaró
January 2012,
Methods in molecular biology (Clifton, N.J.),
