Stabilizing ultrathin cryo-sections by freeze-drying. 1978

W Geymayer, and F Grasenick, and Y Hödl

Cryo-sectioning of specimens with high water content often proves to be especially difficult, as the sections of freeze-shocked samples with their minimal thermal capacity warm up easily. The resultant recrystallization of the included water causes considerable damage in the structure of the frozen specimens. The method described in this paper enables cryo-sections to be transferred from the microtome to a precooled high vacuum plant without contamination and at a temperature below the recrystallization point of ice. In this device the cold sections can be stabilized by freeze-drying, sandwiching or electron-bombardment for subsequent observation in the transmission electron microscope.

UI MeSH Term Description Entries
D008867 Microtomy The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness. Thin Sectioning,Ultramicrotomy,Sectioning, Thin,Sectionings, Thin,Thin Sectionings
D009348 Nematoda A phylum of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites. Phasmidia,Secernentea,Sipunculida
D005612 Freeze Drying Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products. Lyophilization,Drying, Freeze,Dryings, Freeze,Freeze Dryings,Lyophilizations
D005629 Frozen Sections Thinly cut sections of frozen tissue specimens prepared with a cryostat or freezing microtome. Frozen Section,Section, Frozen,Sections, Frozen
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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