-transparent.png){kind=logo}
The chick oviduct cytosol progesterone receptor can be transformed to a small form (Rs = 21A, S20,w:2.9) denoted "mero-receptor" by incubation in the presence of Ca2+ [8]. In the molybdate-free cytosol all the progestin binding components could be completely transformed to mero-form by 1 h treatment with 100 mM Ca2+ at 0 degrees C. If EDTA was secondarily added, the ligand was rapidly released. If molybdate (20 mM) containing cytosol was incubated with Ca2+, no radioactivity was found in the meroposition on the Agarose A 0.5 m column, but the bound steroid sedimented at 2.9 S in sucrose gradients containing Ca2+ (and no molybdate). When 20 nM molybdate was added to cytosol containing receptor activated by 0.3 M KCl, complete mero-transformation by Ca2+ was obtained also by the gel filtration criterion, indicating that molybdate does not inhibit the mero-transforming factor. Ligand-free progesterone receptor could also be completely converted to mero-form by endogenous cytosolic transforming factor and calcium. The transforming factor was completely inactivated, when cytosol was run through Agarose A 0.5 m gel. Mero-transformation was found to be irreversible. The purified progesterone receptor subunit 110 K (B) was partially converted to smaller forms by calcium alone (100 mM, 0 degrees C, 1 h) whereas addition of a small amount of cytosol allowed complete conversion to mero-form.
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
July 1983,
Biochemical and biophysical research communications,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
December 1981,
Journal of steroid biochemistry,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
August 1984,
Molecular and cellular endocrinology,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
January 1986,
Advances in experimental medicine and biology,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
January 1977,
The Journal of biological chemistry,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
June 1990,
Cell differentiation and development : the official journal of the International Society of Developmental Biologists,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
October 1976,
Journal of steroid biochemistry,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
April 1981,
Biochemistry,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
August 1987,
Molecular and cellular endocrinology,
P Tuohimaa, and
J Mester, and
J M Renoir, and
M G Catelli, and
N Binart, and
T Buchou, and
E E Baulieu
January 1987,
Journal of steroid biochemistry,
