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The activity of cyclic AMP-dependent protein kinase (cyclic AMP-PK) was significantly higher (P less than 0.001) in thioglycollate-elicited than in resident rat peritoneal macrophages. The activity ratio of the enzyme (its activity in the absence of added cyclic AMP divided by that in the presence of 5 microM cyclic AMP) was similar in the two cell types. The divalent ion ionophore A23187 induced a rapid increase in the activity ratio of cyclic AMP-PK in both macrophage types. This effect was blocked by pretreating the cells with indomethacin or aspirin (inhibitors of cyclo-oxygenase) and bromo-phenacyl bromide (an inhibitor of phospholipase A2), implicating the synthesis of a prostanoid as an intermediary step. Prostaglandin (PG) E2, 8-bromo cyclic AMP and cholera toxin, all of which inhibit chemiluminescence and/or PG formation in macrophages, increased the activity ratio of cyclic AMP-PK in these cells. We propose that the activation of cyclic AMP-PK plays a central role in the response of macrophages to both endogenously-generated and exogenously added PGE.
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
July 1999,
Molecular and cellular biology,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
October 1983,
FEBS letters,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
November 1981,
FEBS letters,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
June 2012,
Infection and immunity,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
January 1983,
FEBS letters,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
November 1983,
Archives of biochemistry and biophysics,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
January 1984,
Peptides,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
April 1982,
Archives of biochemistry and biophysics,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
December 1982,
Nihon Sanka Fujinka Gakkai zasshi,
N H Hunt, and
L K Lim, and
R D Eichner, and
G D Buffinton, and
M J Weidemann
May 1989,
The Biochemical journal,
