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We have designed and constructed a 25 mm diameter chamber in order to study the phosphorus nuclear magnetic resonance (31P NMR) spectra from a considerable mass of toad and frog muscles (16 sartorii weighing 5-10 g) which were maintained in a well-oxygenated condition at 4 degrees C. We have thus been able to measure the biochemical changes that accompany contraction and recovery with improved time-resolution. Using this apparatus it is shown that splitting of phosphocreatine (PCr) continues for a few minutes after relaxation. Subsequently the PCr is rebuilt by oxidative processes in the familiar way, with a time-constant congruent to 10 min. By studying tetanic contractions of various durations we have shown that the time-course of the post-contractile PCr splitting is similar to that of the heat production that cannot yet be accounted for by known chemical changes. Myosin and actomyosin ATPase reactions most likely underlie the post-contractile ATP utilization. The results suggest that the post-contractile ATP utilization is responsible for the unexplained enthalpy mentioned above.
K Yamada, and
M Tanokura
January 1994,
European journal of applied physiology and occupational physiology,
K Yamada, and
M Tanokura
June 1976,
The Journal of physiology,
K Yamada, and
M Tanokura
January 1985,
Current surgery,
K Yamada, and
M Tanokura
February 1983,
The Biochemical journal,
K Yamada, and
M Tanokura
October 1998,
Neuromuscular disorders : NMD,
K Yamada, and
M Tanokura
July 1987,
Annals of neurology,
K Yamada, and
M Tanokura
May 1985,
The Biochemical journal,
K Yamada, and
M Tanokura
October 1977,
Proceedings of the National Academy of Sciences of the United States of America,
K Yamada, and
M Tanokura
January 1984,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
K Yamada, and
M Tanokura
May 1979,
Seikagaku. The Journal of Japanese Biochemical Society,
