Biomaterial Database

The appearance of fluorescein-labelled lymphocytes in lymph following in vitro or in vivo labelling: the route of lymphocyte recirculation through mesenteric lymph nodes. 1984

G W Chin, and N P Cahill

Fluorescein isothiocyanate (FITC) has been used to study lymphocyte migration in sheep. After being labelled in vitro with FITC, lymphocytes migrated from blood into lymph at the same rate and with the same recovery as lymphocytes labelled with with the radioisotope 51chromium. The in vivo labelling of mesenteric lymph nodes (MLN) with FITC resulted in high numbers of labelled lymphocytes appearing in prescapular lymph. However, the appearance of the FITC-labelled lymphocytes in the prescapular lymph could be prevented by cannulating the main intestinal lymph duct prior to the in vivo labelling procedure. It was concluded that lymphocytes labelled in vivo within the MLN required an intact lymphatic system to reach the blood circulation and did not enter the venous circulation directly from the MLN.

UI	MeSH Term	Description	Entries
D008196	Lymph	The interstitial fluid that is in the LYMPHATIC SYSTEM.	Lymphs
D008198	Lymph Nodes	They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.	Lymph Node,Node, Lymph,Nodes, Lymph
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008643	Mesentery	A layer of the peritoneum which attaches the abdominal viscera to the ABDOMINAL WALL and conveys their blood vessels and nerves.	Mesenteries
D002465	Cell Movement	The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.	Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005260	Female		Females
D005452	Fluoresceins	A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.	Tetraiodofluorescein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D013861	Thiocyanates	Organic derivatives of thiocyanic acid which contain the general formula R-SCN.	Rhodanate,Rhodanates