-transparent.png){kind=logo}
We developed a useful method for the establishment of stable cell lines producing human monoclonal anti-DNA antibody by in vitro Epstein-Barr virus infection. The practical limitation for the cloning was overcome by 2 procedures. One was a microculture system using a small number of the culture. Another was enrichment of anti-DNA producing cells at an early stage and prior to the cloning. The combination of these procedures allowed ready derivation of the cell lines secreting monoclonal anti-DNA antibody. Sixteen cell lines were cloned by utilizing colony formation methods in soft agarose. About 14-32 micrograms per ml of IgM with specific antibody activity were obtained in the supernatant of the cells. The antibody reacted with double-stranded and/or single-stranded DNA. These cells have been continuously producing the specific antibody for more than 3 years. We may extend this procedure for obtaining other autoantibodies, such as anti-T cell antibodies.
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
September 1986,
Immunology letters,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
August 2013,
Journal of microbiology and biotechnology,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
March 1990,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
December 1990,
European journal of epidemiology,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
June 2010,
Journal of biotechnology,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
October 2016,
Monoclonal antibodies in immunodiagnosis and immunotherapy,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
October 1992,
Human antibodies and hybridomas,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
August 1994,
Rinsho kyobu geka = Japanese annals of thoracic surgery,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
October 1990,
Japanese journal of cancer research : Gann,
T Sasaki, and
F Endo, and
M Mikami, and
Y Sekiguchi, and
K Tada, and
Y Ono, and
N Ishida, and
K Yoshinaga
January 2009,
Methods in molecular biology (Clifton, N.J.),
