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The phage phi 29 protein p2, required for the formation of the protein p3-dAMP initiation complex, has been purified from Escherichia coli cells harboring a gene 2-containing recombinant plasmid. The purified protein p2, of molecular weight 68,000, had a specific DNA polymerase activity that elongated the p3-dAMP initiation complex when phi 29 DNA-protein p3 was used as template. In addition, the purified protein p2 was active in catalyzing the initiation reaction when complemented with phi 29 mutant sus2-infected Bacillus subtilis or plasmid-containing E. coli extracts providing protein p3, in the presence of phi 29 DNA-protein p3 as template. However, when purified protein p3 was used in the complementation assay, a very low amount of initiation complex was formed; addition of extracts from uninfected B. subtilis or E. coli strongly stimulated the initiation reaction, indicating that, in addition to proteins p2 and p3 and the phi 29 DNA-protein p3 template, some host factor(s) is required for the formation of the p3-dAMP initiation complex. The results show that phage phi 29 encodes a DNA polymerase that is required at the initiation step of protein-primed DNA synthesis.
L Blanco, and
M Salas
July 1983,
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L Blanco, and
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December 1986,
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L Blanco, and
M Salas
May 1989,
Nucleic acids research,
L Blanco, and
M Salas
November 1983,
Nucleic acids research,
L Blanco, and
M Salas
December 1983,
Nucleic acids research,
L Blanco, and
M Salas
March 1993,
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L Blanco, and
M Salas
December 1982,
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L Blanco, and
M Salas
June 1989,
Nucleic acids research,
L Blanco, and
M Salas
January 1983,
Cold Spring Harbor symposia on quantitative biology,
L Blanco, and
M Salas
January 1988,
Gene,
