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Stereological techniques on electron microscopy micrographs were used to evaluate the morphological changes of cultured islet beta cells that had been exposed to alloxan or 6-hydroxydopamine. Trypan Blue exclusion by cells cultured for 3 days indicated that the cells were 100% viable. Electron microscopy revealed that nearly all of the surviving cultured cells were beta cells. Exposure to 5 mmol/l alloxan or 1-5 mmol/l 6-hydroxydopamine for 10 or 30 min caused a general swelling of the cultured cells with a concomitant swelling of mitochondria and nuclei. The size of the secretory granules was not affected by the drugs. Only 3-10% of the cells excluded Trypan Blue after exposure to 5 mmol/l alloxan or 6-hydroxydopamine. The data conform with the hypothesis that a primary action of alloxan and 6-hydroxydopamine is at the plasma membrane level of beta cells.
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K Grankvist, and
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I B Täljedal
September 1990,
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R Norlund, and
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October 1982,
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I B Täljedal
January 1974,
Hormone research,
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I B Täljedal
June 2001,
General and comparative endocrinology,
R Norlund, and
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Molecular biology of the cell,
R Norlund, and
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I B Täljedal
June 1967,
Diabetologia,
R Norlund, and
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March 1962,
Archives of pathology,
