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The activation of muscle phosphorylase kinase b by actin has been studied. F-actin which is polymerized by 2 mM MgCl2 is a more effective activator of phosphorylase kinase than F-actin polymerized by 50 mM KCl. There is evidence suggesting that the activation of phosphorylase kinase by actin is not due to trace contamination of actin preparations with calmodulin: (1) Troponin I and trifluoperazine inhibit the activation of phosphorylase kinase by calmodulin but do not inhibit the activation of phosphorylase kinase by F-actin. (2) The activation induced by saturating concentrations of calmodulin and actin is additive both at pH 8.2 and at pH 6.8. (3) The activation of phosphorylase kinase by calmodulin and actin has different pH profiles. An addition of F-actin does not affect the apparent Km value for ATP but increases the sensitivity to phosphorylase b and the value of Vmax.
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
August 1981,
Cell calcium,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
August 1979,
FEBS letters,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
January 1981,
Biochimica et biophysica acta,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
April 1971,
The Journal of biological chemistry,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
October 1979,
European journal of biochemistry,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
March 1980,
Biochimica et biophysica acta,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
September 1979,
FEBS letters,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
October 1982,
Circulation research,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
December 1980,
The Journal of biological chemistry,
N B Livanova, and
G V Silonova, and
N V Solovyeva, and
I E Andreeva, and
M V Ostrovskaya, and
B F Poglazov
February 1975,
FEBS letters,
