Biomaterial Database

Idiotypic analysis of anti-I-Ak monoclonal antibodies. I. Production and characterization of syngeneic anti-idiotypic mAb against an anti-I-Ak mAb. 1984

M L Phillips, and J F Harris, and T L Delovitch

To analyze the idiotype (Id) of anti-Ia antibodies elicited during alloimmune responses, we produced syngeneic mouse anti-Id monoclonal antibodies (mAb) reactive with the Id of the 11-5.2.1.9 (11-5) mouse anti-I-Ak (BALB/c anti-CKB) mAb. Two such anti-Id mAb, IA2 (IgG2a) and IIID1 (IgG1), detect structurally related idiotopes located within the binding site of 11-5 for I-Ak antigens. A third anti-Id mAb, VC6 (IgG1), detects an idiotope located either inside or outside of, but presumably proximal to, the 11-5 antigen-binding site, because its expression correlates with the antigenic specificity of 11-5. None of the idiotopes detectable by these three anti-Id mAb are accessible when the binding site of 11-5 is occupied by an I-Ak molecule. The association constants of these anti-Id mAb for their cognate Fab-linked Id range from 2 X 10(9) to 1 X 10(10) M-1. The three anti-Id-producing hybridomas were found with a frequency of 0.008% among growing hybrid colonies. Even though these anti-Id mAb detect public idiotopes (IdX) on 11-5, they do not detect the presence of such IdX markers in the sera of five syngeneic BALB/c mice hyperimmunized with C3H (I-Ak) spleen cells. This suggests that 11-5 represents a BALB/c idiotype infrequently expressed by serum immunoglobulins. The 11-5 idiotopes detectable by IA2, IIID1, and VC6 seem to be conformationally determined by the interaction of 11-5 H and L chains and are not confined to one or the other of these subunit polypeptides. Thus, the expression of the 11-5 Id may be regulated by both VH and VL genes.

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D007130	Immunoglobulin Idiotypes	Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.	Idiotypes, Immunoglobulin,Ig Idiotypes,Idiotype, Ig,Idiotype, Immunoglobulin,Idiotypes, Ig,Ig Idiotype,Immunoglobulin Idiotype
D007143	Immunoglobulin Heavy Chains	The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.	Immunoglobulins, Heavy-Chain,Heavy-Chain Immunoglobulins,Ig Heavy Chains,Immunoglobulin Heavy Chain,Immunoglobulin Heavy Chain Subgroup VH-I,Immunoglobulin Heavy Chain Subgroup VH-III,Heavy Chain Immunoglobulins,Heavy Chain, Immunoglobulin,Heavy Chains, Ig,Heavy Chains, Immunoglobulin,Immunoglobulin Heavy Chain Subgroup VH I,Immunoglobulin Heavy Chain Subgroup VH III,Immunoglobulins, Heavy Chain
D007147	Immunoglobulin Light Chains	Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.	Ig Light Chains,Immunoglobulins, Light-Chain,Immunoglobulin Light Chain,Immunoglobulin Light-Chain,Light-Chain Immunoglobulins,Chains, Ig Light,Chains, Immunoglobulin Light,Immunoglobulins, Light Chain,Light Chain Immunoglobulins,Light Chain, Immunoglobulin,Light Chains, Ig,Light Chains, Immunoglobulin,Light-Chain, Immunoglobulin
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008805	Mice, Inbred A	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	Mouse, Inbred A,Inbred A Mice,Inbred A Mouse
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D006825	Hybridomas	Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.	Hybridoma
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000888	Antibodies, Anti-Idiotypic	Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.	Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies