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Soluble purified monoclonal and polyclonal rheumatoid factor, total serum complement, and soluble C1q all inhibit the detection of model tetanus toxoid/anti-toxoid immune complexes in the solid-phase C1q assay. The binding of these immune complexes to solid-phase monoclonal rheumatoid factor is less inhibited by soluble C1q and by total serum complement, but clearly decreased by soluble monoclonal or polyclonal rheumatoid factor. Serum complement does not reduce the size of these model complexes. We recommend the use of low ionic strength EDTA (10 mM) to partly neutralize the complement-mediated inhibition. This procedure is shown to be superior to currently used higher EDTA concentrations and to the use of IgG-Sepharose.
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
July 1981,
Clinical and experimental immunology,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
January 1981,
Journal of immunological methods,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
December 1979,
The Journal of clinical investigation,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
March 1981,
International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
June 1983,
Clinical and experimental immunology,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
May 1982,
The Journal of laboratory and clinical medicine,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
March 1984,
American journal of clinical pathology,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
June 1976,
Clinical and experimental immunology,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
August 1981,
The British journal of dermatology,
G Vanham, and
F J Bloemmen, and
J L Ceuppens, and
E A Stevens
September 1983,
American journal of veterinary research,
