-transparent.png){kind=logo}
Strong chemical evidence for the existence of a covalent binary complex between 5-fluorodeoxyuridylate and thymidylate synthetase was provided by the isolation of the complex by trichloroacetic acid precipitation. This result together with that of a control experiment with N-ethymaleimide inactivated thymidylate synthetase demonstrated that only nucleotide covalently bound to the protein survived repeated washings of the precipitate. Under the conditions used, a maximum binding stoichiometry of about 0.9 was obtained for the covalent binary complex, Kd = 1.1 X 10(-5) M. Also, a binding ratio of 1.7 was obtained for the methylenetetrahydrofolate-5-fluorodeoxyuridylate-thymidylate synthetase ternary complex.
M A Moore, and
F Ahmed, and
R B Dunlap
August 1978,
Biochemical and biophysical research communications,
M A Moore, and
F Ahmed, and
R B Dunlap
August 1981,
Archives of biochemistry and biophysics,
M A Moore, and
F Ahmed, and
R B Dunlap
January 1980,
Biochemistry,
M A Moore, and
F Ahmed, and
R B Dunlap
April 1982,
European journal of cancer & clinical oncology,
M A Moore, and
F Ahmed, and
R B Dunlap
January 1974,
Biochemistry,
M A Moore, and
F Ahmed, and
R B Dunlap
May 1990,
Analytical biochemistry,
M A Moore, and
F Ahmed, and
R B Dunlap
July 1972,
Proceedings of the National Academy of Sciences of the United States of America,
M A Moore, and
F Ahmed, and
R B Dunlap
January 1980,
Enzyme,
M A Moore, and
F Ahmed, and
R B Dunlap
February 1981,
Biochemical pharmacology,
M A Moore, and
F Ahmed, and
R B Dunlap
September 1989,
Biochemical pharmacology,
