-transparent.png){kind=logo}
A method has been developed for the isolation of islets of Langerhans from the human pancreas. The average number of islets isolated was 1011 islets per gram of pancreas (SD 475, range 752-2111), and the purity of the preparation as defined by histologic examination and specific staining for insulin varied from 10% to 40%. Islet structure was well preserved and the islets were shown to be viable by supravital staining, demonstration of insulin response to glucose, and by transplantation of isolated islets beneath the renal capsule of nude mice. The essential features of this technique for isolation of human islets include injection of a high concentration of collagenase (6 mg/ml) into the pancreatic duct under pressure, followed by a short incubation (23 min) at 39 degrees C. The gland is then dispersed by a process of teasing and shaking, and the islets are separated by a two-stage process of filtration on a nylon mesh to remove the larger islets and centrifugation on a preformed Ficoll density gradient to separate the small islets.
D W Gray, and
P McShane, and
A Grant, and
P J Morris
January 1990,
Voprosy meditsinskoi khimii,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
January 1967,
Diabetes,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
March 1970,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
January 2003,
Methods in molecular medicine,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
June 1994,
Transplantation proceedings,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
March 2008,
Transplantation proceedings,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
August 1988,
Transplantation proceedings,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
July 1989,
Diabetes,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
December 2008,
Transplantation proceedings,
D W Gray, and
P McShane, and
A Grant, and
P J Morris
November 1998,
General and comparative endocrinology,
