BIOMAT Database Logo BIOMAT Database Logo

Serological confirmation of Brucella abortus and Yersinia enterocolitica O:9 O-antigens by monoclonal antibodies. 1984

D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky

Murine monoclonal antibodies that bind the O-antigens of Yersinia enterocolitica serotype O:9 and Brucella abortus 1119-3 were generated after immunization of BALB/c mice with killed, whole cells. Highly purified lipopolysaccharide preparations from each organism were used to screen for antigen-specific antibodies. Immunization with B. abortus cells induced 56 antigen-specific hybrids, and 10 of the highest antibody-producing clones were selected for further study. Seven of these clones secreted immunoglobulin G, and three secreted immunoglobulin M antibodies. Immunization with Y. enterocolitica cells resulted after fusion in 76 antigen-specific hybrid cell lines; from these, seven immunoglobulin G-secreting clones were selected for study. The serological cross-reactivity of the B. abortus and Y. enterocolitica O-antigens was established by enzyme-linked immunosorbent assay, immunoprecipitation, and agglutination tests with the monoclonal antibodies induced by each bacterium. This serological cross-reactivity is consistent with the structural identity of the two O-antigens established by chemical analysis.

UI MeSH Term Description Entries
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D002003 Brucella abortus A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected. Bacterium abortus,Brucella melitensis biovar abortus
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D015008 Yersinia enterocolitica A species of the genus YERSINIA, isolated from both man and animal. It is a frequent cause of bacterial gastroenteritis in children. Bacterium enterocoliticum

Related Publications

D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
The serological response of cattle immunized with Yersinia enterocolitica O:9 or O:16 to Yersinia and Brucella abortus antigens in enzyme immunoassays.
April 1990, Veterinary immunology and immunopathology,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Serological cross-reactions between Brucella abortus and Yersinia enterocolitica 0:9.
September 1985, International journal of zoonoses,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Immunological characterization of Yersinia enterocolitica O:9 and O:3 LPS antigens by monoclonal antibodies.
November 1988, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Biochemical basis of the serological cross-reactions between Brucella abortus and Yersinia enterocolitica serotype O:9.
December 1975, Annales de microbiologie,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7.
May 2004, Veterinary microbiology,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Serological cross-reactions between Brucella abortus and Yersinia enterocolitica IX.
January 1974, Journal of hygiene, epidemiology, microbiology, and immunology,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 I immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis.
December 1995, Veterinary microbiology,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Experimental studies on the serological relationships between Yersinia enterocolitica and Brucella abortus.
November 1979, Research in veterinary science,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Analysis of interferon-gamma producing cells during infections by Yersinia enterocolitica O:9 and Brucella abortus in cattle.
June 2019, Veterinaria italiana,
D R Bundle, and M A Gidney, and M B Perry, and J R Duncan, and J W Cherwonogrodzky
Afipia clevelandensis antibodies and cross-reactivity with Brucella spp. and Yersinia enterocolitica O:9.
November 1997, Clinical and diagnostic laboratory immunology,
Copied contents to your clipboard!