Biomaterial Database

Rapid diagnosis of chlamydial infections with the MicroTrak direct test. 1984

C T Uyeda, and P Welborn, and N Ellison-Birang, and K Shunk, and B Tsaouse

A direct test on clinical specimens, using fluorescein-labeled monoclonal antibodies, for Chlamydia trachomatis (MicroTrak [Syva Co.]) was evaluated for the rapid diagnosis of chlamydial infections. Asymptomatic females attending pregnancy or planned parenthood clinics were tested by the direct test and by a cell culture method. Of 401 paired, endocervical specimens, 398 (99.3%) gave identical direct and culture results. The overall sensitivity of the direct test was 96.3% (26 of 27), and the specificity was 99.5% (372 of 374) as compared with that of culture. More than four-fifths of the direct smears were read within ca. 2 min. In this study population, the performance of the direct specimen test was comparable to that of cell culture methods. Rapid turnaround time and elimination of the need for cell culture make the direct test a practical method for the specific diagnosis of chlamydial infections.

UI	MeSH Term	Description	Entries
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002690	Chlamydia Infections	Infections with bacteria of the genus CHLAMYDIA.	Infections, Chlamydia,Chlamydia Infection,Infection, Chlamydia
D005260	Female		Females
D005452	Fluoresceins	A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.	Tetraiodofluorescein
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D013861	Thiocyanates	Organic derivatives of thiocyanic acid which contain the general formula R-SCN.	Rhodanate,Rhodanates
D014626	Vaginal Smears	Collection of pooled secretions of the posterior vaginal fornix for cytologic examination.	Cervical Smears,Cervical Smear,Smear, Cervical,Smear, Vaginal,Smears, Cervical,Smears, Vaginal,Vaginal Smear