Biomaterial Database

Detection of serum antibody to Brucella abortus in cattle by use of a quantitative fluorometric immunoassay. 1984

S M Hall, and A W Confer, and L B Tabatabai, and B L Deyoe

A quantitative fluorometric immunoassay (FIAX) was adapted for the detection of serum antibodies to Brucella abortus in cattle. Results are expressed in nanograms of immunoglobulin binding the antigen carrier. The FIAX was compared with the standard tube agglutination, Rivanol precipitation, and complement fixation tests, using 285 serum samples from vaccinated, challenged, or control cattle. Linear regression analysis indicated a significant correlation among all four serological tests; the FIAX test correlated best with the Rivanol test. Ninety sera were from vaccinated and nonvaccinated cattle that were challenged with virulent B. abortus 2308. The sensitivity and specificity of each serological test were determined based on culture results from these cattle. The FIAX was the most sensitive of the four serological tests, detecting 79.2% of the culture-positive animals. The FIAX was the least specific, with 15.4% of the culture-negative animals being classified as positive. Eighty-eight sera were from cattle vaccinated with strain 19 but not challenged. All four serological tests had a statistically significant ability to distinguish sera from control and vaccinates on the basis of mean titers. The mean titer of vaccinates was also significantly different from that of challenged animals. Advantages and disadvantages of the FIAX test for bovine brucellosis are discussed.

UI	MeSH Term	Description	Entries
D002003	Brucella abortus	A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.	Bacterium abortus,Brucella melitensis biovar abortus
D002007	Brucellosis, Bovine	A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.	Bang's Disease,Bang Disease,Bangs Disease,Bovine Brucelloses,Bovine Brucellosis,Brucelloses, Bovine,Disease, Bang,Disease, Bang's
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies