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In a prospective study of 38 patients who presented with hematuria of renal origin, 15 patients were found to have primary IgA nephropathy and 23 had other renal disorders. Sera and renal biopsy specimens of these patients were studied for the presence of macromolecular IgA1 and IgA2 using monoclonal antibodies, and the presence of J-chain as demonstrated either by immunofluorescence or its capacity to bind free secretory component. Circulating macromolecular IgA was found exclusively in the sera of patients (80%) with primary IgA nephropathy. In these sera the polymer/monomer ratio for IgA1 (0.64 +/- 0.13) was significantly higher than for normal human serum (0.39 +/- 0.01) (P less than 0.001), while no differences were found for IgA2. The polymeric IgA1 was isolated from serum by gel chromatography and was shown to have the capacity to bind free secretory component. Direct two-color immunofluorescence studies revealed the presence of only IgA1 in the mesangial deposits and also its capacity to bind free secretory component. We conclude (1) that demonstration of circulating macromolecular IgA in patients with renal hematuria is of diagnostic value and (2) that antigenetic similarities between the circulating and the mesangial macromolecular IgA suggest that dimeric IgA1 is deposited in the mesangium of patients with primary IgA nephropathy.
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1987,
Advances in experimental medicine and biology,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1984,
Contributions to nephrology,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1995,
Nephron,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
March 1982,
Clinical and experimental immunology,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 2021,
Kidney & blood pressure research,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1990,
Revista do Hospital das Clinicas,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
September 1983,
American journal of kidney diseases : the official journal of the National Kidney Foundation,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1985,
British medical journal (Clinical research ed.),
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1984,
Contributions to nephrology,
R M Valentijn, and
J Radl, and
J J Haaijman, and
B J Vermeer, and
J J Weening, and
R H Kauffmann, and
M R Daha, and
L A van Es
January 1989,
Nephron,
